MICROBIOLOGICAL TRANSFORMATION OF NITROGE N-CONTAINING HETEROCYCLIC-COMPOUNDS

Pyridine-utilizing microorganisms were isolated from soil using accumu lative cultivation, They were identified as Arthrobacter crystallopoie tes VKM Ac-1334D and Rhodococcus opacus VKM Ac-1333D. Gas chromatograp hy and mass spectroscopy showed that the former strain growing on pyri dine produces intermediate 3-hydroxypyridine and 2,3- and 2,6-dihydrox ypyridines, while the latter strain yields 2-hydroxypyridine and 2,6-d ihydroxypyridine as intermediate products. In addition, these strains perform specific ring cleavage leading to 5-amino-2-oxopent-4-enoic ac id and 4-carbamoylbut-3-enoic acid, respectively.