NITRIC-OXIDE PLAYS NO ROLE IN ACTH RELEASE INDUCED BY INTERLEUKIN-1-BETA CORTICOTROPIN-RELEASING HORMONE, ARGININE-VASOPRESSIN AND PHORBOL-MYRISTATE ACETATE IN RAT PITUITARY CELL-CULTURES
K. Hashimoto et al., NITRIC-OXIDE PLAYS NO ROLE IN ACTH RELEASE INDUCED BY INTERLEUKIN-1-BETA CORTICOTROPIN-RELEASING HORMONE, ARGININE-VASOPRESSIN AND PHORBOL-MYRISTATE ACETATE IN RAT PITUITARY CELL-CULTURES, Endocrine journal, 42(3), 1995, pp. 435-439
To investigate the role of nitric oxide (NO) in pituitary ACTH secreti
on,the effect of a nitric oxide synthase (NOS) inhibitor, N omega-Nitr
o-L-arginine (Nitro-Arg), on ACTH secretion induced by interleukin (IL
)-1 beta, corticotropin releasing hormone (CRH), arginine vasopressin
(AVP) and phorbol myristate acetate (PMA) was examined in rat anterior
pituitary cell cultures. Nitro-Arg did not affect IL-1 beta-induced A
CTH release in pituitary cell cutures incubated with Dulbecco modified
Eagle's medium, Basal Medium Eagle or Krebs Ringer bicarbonate-glucos
e buffer. It did not affect CRH-, AVP- or PMA-induced ACTH release eit
her. These results suggest that NO does not play an important role in
ACTH release at the pituitary level.