COMPOUNDS 2,4,6-TRINITROTOLUENE AND 2,4-D IAMINO-6-NITROTOLUENE - THEABSENCE OF RECA-DEPENDENT MUTAGENESIS

Citation
Ns. Karamova et al., COMPOUNDS 2,4,6-TRINITROTOLUENE AND 2,4-D IAMINO-6-NITROTOLUENE - THEABSENCE OF RECA-DEPENDENT MUTAGENESIS, Genetika, 31(5), 1995, pp. 617-621
Citations number
25
Categorie Soggetti
Genetics & Heredity
Journal title
ISSN journal
00166758
Volume
31
Issue
5
Year of publication
1995
Pages
617 - 621
Database
ISI
SICI code
0016-6758(1995)31:5<617:C2A2I->2.0.ZU;2-E
Abstract
The genotoxicity of 2,4,6-trinitrotoluene (2,4,6-TNT) and its amino de rivative, 2,4-diamino-6-nitrotoluene (2,4-DA-6-NT), was studied using the Escherichia coil tester strain PQ37 in the SOS chromotest. The com pound 2,4,6-TNT, without metabolic activation, virtually failed to ind uce an SOS effect in cells of the tester bacteria. Consequently, mutag enic activity of 2,4,6-TNT, which was shown earlier in the Ames test, does not depend on SOS mutagenesis. It was demonstrated that metabolic activation with the microsomal S9 human placenta fraction results in a threefold increase in the induction factor of the SOS effect caused by 2,4,6-TNT. The absence of the SOS-inducing activity of 2,4-DA-6-NT, regardless of the presence of a microsomal activating mixture is show n. Thus, 2,4-DA-6-NT does not belong to metabolites of 2,4,6-TNT, resp onsible for the genotoxicity of this compound.