Ns. Karamova et al., COMPOUNDS 2,4,6-TRINITROTOLUENE AND 2,4-D IAMINO-6-NITROTOLUENE - THEABSENCE OF RECA-DEPENDENT MUTAGENESIS, Genetika, 31(5), 1995, pp. 617-621
The genotoxicity of 2,4,6-trinitrotoluene (2,4,6-TNT) and its amino de
rivative, 2,4-diamino-6-nitrotoluene (2,4-DA-6-NT), was studied using
the Escherichia coil tester strain PQ37 in the SOS chromotest. The com
pound 2,4,6-TNT, without metabolic activation, virtually failed to ind
uce an SOS effect in cells of the tester bacteria. Consequently, mutag
enic activity of 2,4,6-TNT, which was shown earlier in the Ames test,
does not depend on SOS mutagenesis. It was demonstrated that metabolic
activation with the microsomal S9 human placenta fraction results in
a threefold increase in the induction factor of the SOS effect caused
by 2,4,6-TNT. The absence of the SOS-inducing activity of 2,4-DA-6-NT,
regardless of the presence of a microsomal activating mixture is show
n. Thus, 2,4-DA-6-NT does not belong to metabolites of 2,4,6-TNT, resp
onsible for the genotoxicity of this compound.