EFFECTS OF ACTION OF EXOGENOUS DNA IN TOM ATO .1. GENETIC-TRANSFORMATION

An alien gene for neomycin phosphotransferase II (NPT II) was transfer red into intact tomato plants by means of pollination and fertilizatio n. Nine resistant genotypes of Lycopersion esculentum x Solanum pennel lii were isolated. In all hybrids selected in vitro as resistant to ka namycin, activity of the alien gene NPT II was revealed. The presence of this gene in the genome of plants was shown by dot hybridization wi th a radioactively labeled probe containing the sequence of the NPT II gene. Inheritance of the trait of resistance fo kanamycin was traced in the second generation. The character of inheritance was investigate d on different levels: molecular, phenotypic, gametophytic, and sporop hytic.