INSERTIONAL SEQUENCE PRIMERS FOR BORDETELLA-PERTUSSIS DIAGNOSTIC POLYMERASE CHAIN-REACTION DIFFERENTIATE STRAINS OF PSEUDOMONAS-CEPACIA

Authors
CIMOLAI N TROMBLEY C
Citation
N. Cimolai et C. Trombley, INSERTIONAL SEQUENCE PRIMERS FOR BORDETELLA-PERTUSSIS DIAGNOSTIC POLYMERASE CHAIN-REACTION DIFFERENTIATE STRAINS OF PSEUDOMONAS-CEPACIA, The Journal of infectious diseases, 172(1), 1995, pp. 293-295
Citations number
11
Categorie Soggetti
Infectious Diseases
Journal title
The Journal of infectious diseases → ACNP
ISSN journal
00221899
Volume
172
Issue
1
Year of publication
1995
Pages
293 - 295
Database
ISI
SICI code
0022-1899(1995)172:1<293:ISPFBD>2.0.ZU;2-8
Abstract
Insertion sequence primers originally intended to amplify singular spe cific product for the rapid diagnosis of Bordetella pertussis respirat ory infection were used to differentiate strains fo Pseudomonas (Burkh olderia) cepacia. A modified sample preparation of proteinase K treatm ent and boiling ws used in lieu of DNA extraction. The method was simp le, rapid and reproducible. This scheme identified 10 variations among 35 strains. Repeat strains from patients with cystic fibrosis and epi demiologically linked strains from an infection associated with a jet gun injection device were homologous in each setting.