ANALYSIS OF CHIMERISM AFTER BONE-MARROW T RANSPLANTATION USING PCR AMPLIFICATION OF MINISATELLITES OR Y-CHROMOSOME MARKER

We used polymerase chain reaction amplification of minisatellites sequ ences (33.6.3, G3, PMS51, YNZ22) and of a Y chromosome specific sequen ce (DYZ1) to document chimaerism in 50 patients. Indications for bone marrow transplantation (BMT) was acute leukemia (n=23). Aplastic anemi a (n=18) and inherited newborn desease, SCID and familial hemophagocyt ic lymphohistiocytosis (n=9). The method was informativ in all cases. We were able to demonstrate engraftment in 23/23 leukemia patients, 15 /18 in aplastic patients and 8/9 in the last group of patients. HLA mi smatching BMT was significantly less efficient. Analysis with DYZ1 pro be in case of sex mismatched. BMT revealed the frequency of host resid ual cells after Bott. Their detection in a 0,01-1 % range does not see ms to influence the outcome of the transplantation procedure.