EFFECT OF INTERFACIAL PRESSURE ON THE BINDING AND PHOSPHOLIPASE-A2 ACTIVITY OF RECOMBINANT HUMAN LECITHIN-CHOLESTEROL ACYLTRANSFERASE

Authors
WEINBERG RB JONES JB PRITCHARD PH LACKO AG
Citation
Rb. Weinberg et al., EFFECT OF INTERFACIAL PRESSURE ON THE BINDING AND PHOSPHOLIPASE-A2 ACTIVITY OF RECOMBINANT HUMAN LECITHIN-CHOLESTEROL ACYLTRANSFERASE, Biochemical and biophysical research communications, 211(3), 1995, pp. 840-846
Citations number
30
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
211
Issue
3
Year of publication
1995
Pages
840 - 846
Database
ISI
SICI code
0006-291X(1995)211:3<840:EOIPOT>2.0.ZU;2-D
Abstract
We examined the effect of surface pressure on the interfacial binding and phospholipase A2 activity of lecithin-cholesterol acyltransferase. The enzyme bound to phosphatidylcholine monolayers with an apparent d issociation constant of 1.5 nM was excluded from the interface at pres sures > 29 mN/m and exhibited maximal phospholipase activity at pressu res between 26-28 mN/m. These data suggest that lipoprotein surface pr essure may regulate lecithin-cholesterol acyltransferase activity in v ivo. (C) 1995 Academic Press, Inc.