DEVELOPMENT AND TESTING OF A NEW COMPUTERIZED METHOD FOR MULTICOMPONENT KINETIC DETERMINATIONS BASED ON MULTIWAVELENGTH SPECTROPHOTOMETRIC DETECTION

We developed a new kinetic multidetermination method based on multiwav elength spectrophotometric detection and used simulated processes to e valuate its performance. The method uses spectral differences between analytes and kinetic equations solved by means of a nonlinear regressi on procedure. It allows the simultaneous determination of rate constan ts and analyte concentrations, and takes account of potential matrix e ffects and changes in the rate constants arising from temperature osci llations. The results obtained by using simulated Gaussian spectra rev ealed that the method allows correct resolution of simultaneous kineti c processes, even if they involve strong spectral overlap and identica l kinetic constants. The ''standardized spectral difference'', concept introduced with this work, allows results to be extrapolated to non-G aussian spectra.