R. Brunkhorst et A. Mahiout, PYRUVATE NEUTRALIZES PERITONEAL DIALYSATE CYTOTOXICITY - MAINTAINED INTEGRITY AND PROLIFERATION OF CULTURED HUMAN MESOTHELIAL CELLS, Kidney international, 48(1), 1995, pp. 177-181
Toxic effects of commercially available peritoneal dialysate (PD) flui
d include damage to mesothelial cells (MC), causing a severely disturb
ed proliferation of cultured MC. We investigated the injury to the cel
l membrane (by release of lactate dehydrogenase, LDH), the proliferati
on (by cell counts and by H-3-thymidine incorporation), and optional t
he cytokine generation (by IL-1 receptor-antagonist production, IL-1 r
a) of cultured human MC during the 48 hours after a 30 minute exposure
to PD containing either 35 mmol/liter sodium lactate or sodium pyruva
te. All solutions had a pH of 5.2 to 5.6 and were composed as standard
PD. Glucose contents of 1.36 and 3.86 mmol/liter were tested. After e
xposure to the lactate-PD containing 1.36% glucose, LDH activity was i
ncreased by more than 30%, proliferation of MC was inhibited by more t
han 30%, and IL-1 ra production was reduced significantly when compare
d to pyruvate-PD and the control solution. After preincubation with 3.
86% glucose containing PD, all negative effects became even more prono
unced in the lactate group whereas the MC maintained their integrity,
rate of proliferation and IL-1 ra release after pre-exposure to pyruva
te containing PD. These results suggest that the acute toxic effects o
f commercially available PD on the integrity, proliferation and IL-1 r
a production of MC can be avoided by the use of sodium pyruvate instea
d of sodium lactate.