IN-VITRO AND IN-VIVO ANTIMICROBIAL ACTION OF FLUPHENAZINE

The antipsychotic drug fluphenazine was obtained in a dry powder form and was screened with respect to 482 strains of bacteria which include d 170 Gram-positive and 326 Grain-negative strains. Nutrient agar plat es containing increasing concentrations of fluphenazine (0-200 mu g/ml ) were used for the determination of the minimum inhibitory concentrat ion (MIG) which was demonstrated by inoculating a loopful of an overni ght peptone water culture of the organism on nutrient agar plates and determining the MIC against a control. Fluphenazine was detected to po ssess pronounced action against both Gram-positive and Gram-negative b acteria at 20-100 mu g/ml. In the in vivo studies it was seen that whe n fluphenazine was used at a concentration of 1.5 mu g/g and 3 mu g/g mouse body weight both the levels offered significant protection to Sw iss strain of white mice when challenged with 50 minimum lethal dose ( MLD) of a virulent strain of Salmonella typhimurium 74. The in vivo da ta with fluphenazine were highly significant (p <0.001) according to t he chi-square test.