Y. Hassan et al., IMMUNE-RESPONSES IN MICE INDUCED BY HSV-1 GLYCOPROTEINS PRESENTED WITH ISCOMS OR NISV DELIVERY SYSTEMS, Vaccine, 14(17-18), 1996, pp. 1581-1589
The purpose of this study was to evaluate the immunogenicity of a herp
es simplex virus type 1 (HSV-1) antigen preparation, obtained followin
g zwitterionic detergent treatment of virus, and incorporation of the
antigens into either immunostimulating complexes (ISCOMs) or non-ionic
surfactant vesicles (NISV) delivery systems. Using Balb/c mice the IS
COM and NISV HSV-1 vaccines were assayed for their capacity to induce
and enhance both the humoral and cellular immune responses, and to eli
cit protection against both homologous and heterologous virus challeng
e. The serum from animals vaccinated with either the NISV or the ISCOM
HSV-1 antigen preparation, were found to contain high levels of total
IgG and IgG1 and IgG2a subclass antibodies. In addition, both prepara
tions were found to induce high neutralizing (NT) antibody levels foll
owing a two immunization protocol and to provide some protection again
st homologous and heterologous HSV challenge infection. Lymphoprolifer
ative responses were observed in cultures of splenocytes from mice imm
unized with both HSV-1 NISV vaccine and HSV-1 ISCOMs vaccine, followin
g various antigenic stimuli in vitro. In general, these were most mark
ed in animals immunized with the HSV-1 NISV preparation, and particula
rly so when the splenocytes were stimulated in vitro with live HSV-1.
Both the NISV and ISCOM HSV-1 vaccines were found to have induced inte
rleukin 2, interleukin 10 and interferon-gamma in spleen cell culture
supernatants, although again, the highest responses in general were ob
served in supernatant fluids from spleen cell cultures from animals im
munized with the HSV-1 NISV preparation. These results suggest that a
wide range of immune activity can be elicited by HSV-1 antigens presen
ted to the immune system of mice in these formulations. Copyright (C)
1996 Elsevier Science Ltd.