AN AUTOMATIC SAMPLING DEVICE FOR CAPILLARY ZONE ELECTROPHORESIS

An automatic sampling device has been developed for capillary zone ele ctrophoresis. The sample is introduced to the column electrokineticall y by rapidly exchanging buffer for sample in a narrow channel drilled in a plexiglass block. The autosampler is capable, under computer cont rol, of performing multiple sample injections from a large volume samp le source (such as a reaction vessel) as the sample concentration chan ges, and thus presents the possibility of analyzing time-varying proce sses by CZE. Peak area reproducibility in electropherograms obtained a fter use of the sampler is less than 1 % and efficiency is more than 2 .2 x 10(5) theoretical plates.