EFFECT OF THYROID-HORMONE ADMINISTRATION ON THE DEPLETION OF CIRCULATING GLUTATHIONE IN THE ISOLATED-PERFUSED RAT-LIVER AND ITS RELATIONSHIP TO BASOLATERAL GAMMA-GLUTAMYL-TRANSFERASE ACTIVITY
La. Videla et V. Fernandez, EFFECT OF THYROID-HORMONE ADMINISTRATION ON THE DEPLETION OF CIRCULATING GLUTATHIONE IN THE ISOLATED-PERFUSED RAT-LIVER AND ITS RELATIONSHIP TO BASOLATERAL GAMMA-GLUTAMYL-TRANSFERASE ACTIVITY, Journal of biochemical toxicology, 10(2), 1995, pp. 69-77
The influence of thyroid hormone administration on liver glutathione (
GSH) extraction in the isolated perfused liver was studied in fed rats
for a period of 1-7 days following a single dose of 0.1 mg 3,5,3'-tri
iodothyronine (T-3)/kg. T-3 treatment led to an early and transient ca
lorigenic response, as well as an enhancement in liver GSH removal, re
aching a maximal effect at 2 days after hormone administration, which
was normalized in the 3- to 7-day period studied. Addition of the gamm
a-glutamyltransferase (gamma-GT) inhibitor DL-serine-borate (4 mM) to
the perfusate abolished the increase in the hepatic removal of GSH eli
cited by T-3, and enhanced the sinusoidal concentration of GSH, studie
d at 2 days after hormone administration. These data support the role
of hepatic basolateral gamma-GT ectoactivity in the depletion of porta
lly added and liver-derived GSH as an adaptive response to recover GSH
levels after reduction by T-3-induced oxidative stress.