Sm. Somani et al., RESPONSES OF ANTIOXIDANT SYSTEM TO ACUTE AND TRAINED EXERCISE IN RAT-HEART SUBCELLULAR-FRACTIONS, Pharmacology, biochemistry and behavior, 51(4), 1995, pp. 627-634
The effects of acute and trained exercise on antioxidant enzymes (AGE)
, glutathione (GSH), and malondialdehyde (MDA) were compared in rat he
art subcellular fractions and red blood cells. Fischer-344 rats were e
xercised acutely to 100% VO2 max and another group of Fischer-344 rats
were given trained exercise for 10 weeks. The AOE and MDA were measur
ed by spectrophotometry and GSH and oxidized GSH (GSSG) by high pressu
re liquid chromatography. Trained exercise significantly increased cyt
osol GSH to 131% of sedentary control (SC). Acute exercise significant
ly increased mitochondrial superoxide dismutase, catalase, and glutath
ione peroxidase by 167%, 358%, and 129% of SC, respectively, whereas e
nzyme activities following trained exercise were increased by 133%, 16
6%, and 128% of SC. The mitochondria/cytosolic ratio for superoxide di
smutase, catalase, and glutathione peroxidase after acute exercise inc
reased to 1.9, 2.7, and 1.7, respectively, whereas the respective rati
os of these enzymes after trained exercise were 1.3, 1.6, and 1.3. Acu
te exercise contributed to oxidative stress more than trained exercise
. Acute exercise resulted in a larger increase in enzyme activities th
an trained exercise, possibly as a compensatory mechanism to cope with
the enhanced production of superoxides and oxyradicals during exhaust
ive exercise.