APPLICATION OF MICROPARTICLE-ENHANCED NEP HELOMETRIC IMMUNOASSAYS OF ALPHA-CASEINS, BETA-CASEINS AND KAPPA-CASEINS TO EVALUATE QUALITY OF MILK, FROM THE PRODUCTION TO THE VALORIZATION IN CHEESE INDUSTRY

Citation
P. Montagne et al., APPLICATION OF MICROPARTICLE-ENHANCED NEP HELOMETRIC IMMUNOASSAYS OF ALPHA-CASEINS, BETA-CASEINS AND KAPPA-CASEINS TO EVALUATE QUALITY OF MILK, FROM THE PRODUCTION TO THE VALORIZATION IN CHEESE INDUSTRY, Le Lait, 75(3), 1995, pp. 211-237
Citations number
68
Categorie Soggetti
Food Science & Tenology
Journal title
ISSN journal
00237302
Volume
75
Issue
3
Year of publication
1995
Pages
211 - 237
Database
ISI
SICI code
0023-7302(1995)75:3<211:AOMNHI>2.0.ZU;2-U
Abstract
alpha-, beta- and kappa-caseins were assayed, using a microparticle-en hanced nephelometric immunoassay, in 1300 milk samples, collected fort nightly during 13 months (october 1992 to october 1993), from 50 herds from Normandie (France). The concentration of these three caseins was compared with those of other milk compounds (protein, fat, lactose an d dry matter). The main factors defining milk composition and related to the farm, herd, herd management and feeding, were then studied. The biochemical composition and rennet-coagulation (rennet coagulation ti me, firming time K15, and firmness parameter A30) were evaluated durin g the same period, in raw and fat-standardized milks, used in an indus trial production of cheese. Performed in short and narrow limits, our work was a global study on milk from the production to the valorizatio n in cheese industry. It allowed to confirm well known and copiously p ublished results concerning milk biochemistry (relations between compo sition parameters, evolution and variability of these parameters), zoo technology (influence of breed, pedologic potentialities of the grazin g soils, seasonal distribution of calving, feeding conditions of trans ition from stabbling to pasture) and cheese-making (relative influence of the parameters of composition on the rennet-coagulability bf milk and cheese-making yields). Microparticle-enhanced nephelometric immuno assay, allowing a specific and accurate assessment of the milk casein, thus appeared as an immunoanalytical method, completing the tradition al tools for the evaluation of milk quality.