A new pressing unit was designed to improve the extraction methodology
of the cheese aqueous phase, originally proposed by Barthel at al (19
28a). Emmental aqueous phases extracted from cheeses at different ripe
ning ages: after brining - before and after the warm room and at comme
rcialization were characterized bacteriologically, physico-chemically
and biochemically. Total solids content which is originally 90 g/kg of
the aqueous phase increases up to 170 g/kg in the fully ripened chees
e phase. Most of this increase in caused by the proteolysis, mainly du
ring the warm room period, which leads to a 5 times higher nitrogen co
ntent. A large part (56%) of the nitrogen water soluble components is
free amino acids, some being at oversaturation (Tyr and Glu). No resid
ual sugar was determined in aqueous phase and 25% of the lactate prese
nt in the brined cheese phase is consumed by propionibacteria during t
he warm room period, mainly as L form. Deep modifications of phosphoca
lcic salts are detected: Ca/P ratio varies between 8.2 and 5.4 in the
aqueous phase instead of 1.3 in milk. Na/Cl ratio also does not agree
with the theoretical value in NaCl molecule, leading to the conclusion
that part of Cl ion is mobilized in other saline forms, maybe chlorhy
drate. Characterization of the cheese aqueous phase appears to be a pr
ivileged methodology to follow precisely the effects of technological
cheesemaking parameters and ripening conditions.