J. Kimura et al., PRODUCTION AND IMMUNOHISTOCHEMICAL CHARACTERIZATION OF SPECIFIC ANTIBODY TO OVIDUCTAL FLUID OF MINIATURE SHIBA GOAT, Journal of veterinary medical science, 57(3), 1995, pp. 427-431
Monoclonal antibodies were produced against the oviductal secretory su
bstances in 'Shiba' goats and their nature was characterized. In the a
mpullary region, very strong immunoreactivity with one clone (EE-1) wa
s localized within the cytoplasm of non-ciliated cells (''secretory ce
lls''), whereas very little or no immunoreactivity was observed in the
isthmus region. This reactivity pattern was observed in both estrous
and luteal phases. Immunohistochemical analysis showed the antigen to
be either absent or minimally present in other organs including the al
imentary canal, liver, pancreas, lung, kidney, uterus, thyroid gland,
heart, spleen and spinal cord, and in oviducts of other species such a
s hamster, sheep and cow. The immunoglobulin isotype of EE-1 was deter
mined as IgM with a kappa light chain. The positive reactions were eli
minated by treating deparaffinized sections with periodic acid or test
icular hyaluronidase. Results obtained in this study suggest that ampu
llary secretory cells in 'Shiba' goat oviduct contain a species- and t
issue-specific antigen containing hyaluronic acid or hyaluronidase-sen
sitive carbohydrates.