Mlg. Romano et L. Berrens, COMPARATIVE-STUDIES OF ALLERGENIC EXTRACTS PREPARED FROM FRESHLY COLLECTED, DRIED, OR DRIED AND DEFATTED POLLEN, Journal of investigational allergology & clinical immunology, 5(3), 1995, pp. 142-147
Fresh pollen samples of Parietaria judaica, Betula pubescens, Dactylis
glomerata and Olea europaea were collected and aliquot portions of th
ese pollens were stored after drying in hot air, while other portions
were dried and then defatted with diethylether. The different pollen m
aterials were extracted with aqueous media to obtain the non-dialyzabl
e allergenic protein constituents. Review of the production data, toge
ther with comparative analyses of the UV-absorption spectra and of IgE
binding potencies did not reveal appreciable differences in allergen
composition among extracts of fresh, dried, or dried and defatted poll
en materials. However, microscopic examination showed the fresh pollen
to be no longer viable.