B. Bouchon et al., MEMBRANE-PROPERTIES OF CULTURED GLIAL-CELLS OBTAINED MECHANICALLY OR BY ENZYMATIC DISSOCIATION - DIFFERENTIAL ALTERATIONS BY GANGLIOSIDES, Journal of neuroscience research, 41(4), 1995, pp. 510-517
Rat astroglial cells were obtained either mechanically or by enzymatic
dissociation and some properties of the primary and secondary culture
s were studied. Differences in the ganglioside amount, taurine uptake,
membrane fluorescence anisotropy, or their respective modulation by t
otal brain gangliosides confirmed that although the two types of cultu
res are morphologically similar, they are biochemically distinct. (C)
1995 Wiley-Liss, Inc.