SYNGENEIC BONE-MARROW TRANSPLANTATION ELIMINATES V-BETA-8.2 T-LYMPHOCYTES FROM THE SPINAL-CORD OF LEWIS RATS WITH EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

Experimental allergic encephalomyelitis (EAE), an animal model for mul tiple sclerosis (MS), is a paralytic disease of the central nervous sy stem (CNS) mediated by T-lymphocytes reactive to myelin basic protein (MBP), Lewis rats actively immunized with fragment 68 to 82 of guinea pig MBP develop a monophasic disease with spontaneous recovery, Lympho cyte recognition of the primary encephalitogenic sequence of MBP (frag ment 68 to 82) is V(beta)8.2 T cell receptor (TCR) skewed [1-3]. Lewis rats in clinical remission at 1 month and 3 months after spontaneous resolution of EAE retain V(beta)8.2 T-lymphocytes in the CNS when anal yzed by reverse transcriptase polymerase chain reaction or in situ hyb ridization, In contrast, 1 and 3 months after clinical remission from syngeneic bone marrow transplantation, V(beta)8.2 T lymphocytes are ab sent from the CNS. During clinically active EAE and inflammatory break down of the blood-brain barrier, immune ablation and reconstitution wi th syngeneic bone marrow results in clinical tolerance of the new immu ne system to myelin. (C) 1995 Wiley-Liss, Inc.