DETECTION OF FOOT-AND-MOUTH-DISEASE VIRUS IN NASAL SWABS OF ASYMPTOMATIC CATTLE BY RT-PCR WITHIN 24 HOURS

Authors
MARQUARDT O STRAUB OC AHL R HAAS B
Citation
O. Marquardt et al., DETECTION OF FOOT-AND-MOUTH-DISEASE VIRUS IN NASAL SWABS OF ASYMPTOMATIC CATTLE BY RT-PCR WITHIN 24 HOURS, Journal of virological methods, 53(2-3), 1995, pp. 255-261
Citations number
22
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
53
Issue
2-3
Year of publication
1995
Pages
255 - 261
Database
ISI
SICI code
0166-0934(1995)53:2-3<255:DOFVIN>2.0.ZU;2-C
Abstract
A method for extracting RNA from animal-derived materials that provide s foot-and-mouth disease viral template suitable for Tth polymerase-de pendent synthesis of cDNA and subsequent PCR is described. Viral genom es were detected in less than 24 h. Nasal swabs that can be easily and repeatedly collected, proved suitable for virus detection by PCR, eve n during the asymptomatic stages of infection.