SECRETION OF NEUTRAL AND ACID DNASES IN CULTIVATED HUMAN-LYMPHOCYTES AFTER INCUBATION WITH DNA - POSSIBLE CONSEQUENCES FOR INHALATION ANESTHESIA

After incubation with DNA human lymphocytes release neutral and acid D Nase activities into the culture medium; the release depends on DNA co ncentration and time of cultivation. The electrophoretic mobility of t he released neutral DNase activity is in accordance with DNase I and t he electrophoretic mobility of the released acid DNase activity with D Nase II. The released DNase activities do not originate from dead cell s and are not influenced by blast cell formation. The anesthetic halot hane can inhibit the released neutral and acid DNase activities. Inhal ation anesthesia can possibly disturb the correlation between DNA and DNases in human blood.