Background and Aims: Infection of rabbits with coccidia (Eimeria magna
) causes chronic ileal inflammation and diarrhea. inflamed ileum also
shows decreased transmural conductance. The aim of this study was to c
haracterize morphological factors known to affect paracellular permeab
ility that may alter transmural conductance in inflamed ileum. Methods
: Ileal mucosa was mounted in Ussing chambers for study of [H-3]mannit
ol and [H-3]inulin fluxes. Light and electron microscopy were used for
morphometric studies. Alterations in the zonula occludens of epitheli
al cells were evaluated in freeze-fracture replicas. Results: Inflamed
ileum showed diminished paracellular fluxes. Inoculated rabbits showe
d marked lymphoplasmocytic infiltration and villus blunting in ileum.
Villus linear junctional density was unaffected. However, total villus
apical surface area per square centimeter of tissue was reduced in in
flamed ileum, causing a diminished total villus linear junctional path
way per square centimeter of apical surface. Villus zonula occludens s
trand number was reduced in inflamed ileum, whereas the frequency of b
oth villus and crypt lateral surface extrajunctional strands increased
. Conclusions: Chronic inflammation exerts a profound effect on ileal
paracellular permeability. Morphological data suggest that this effect
may be caused in part by alterations in inflamed ileal mucosal struct
ure and tight junctional organization and density, particularly on vil
li.