Glucagon-like peptide-1 (7-36)amide has been described as exerting pot
ent glycogenic action and as stimulating glycolysis in skeletal muscle
. We exposed isolated rat soleus muscle strips to various concentratio
ns of glucagon-like peptide-1(7-36) amide (10(-11)-10(-6) mol/l) or in
sulin (10(-10)-10(-7) mol/l) and determined the respective effects on
glucose metabolism. Insulin markedly increased the rate of glucose inc
orporation into glycogen with a maximal effect at 10(-8) mol/l insulin
(348 +/- 46 % of intraindividual control experiment, p < 0.005), whil
e glucagonlike peptide-1 (7-36)amide was without an effect (e.g. 10(-1
1) mol/l, 96 +/- 10 %; 10(-9) mol/l, 104 +/- 9 %: 10(-7) mol/l, 121 +/
- 13 %; not significant). Likewise, glucagon-like peptide-1(7-36)amide
did not affect the rate of H-3-2-deoxy-glucose transport or glycogen
content of soleus muscle strips. The rates of aerobic or anaerobic gly
colysis were also not increased. The findings were independent of pept
ide source and of employed muscle size. Our results do not suggest any
effect of glucagon-like peptide-1(7-36)amide on skeletal muscle gluco
se metabolism and, hence, are in contrast to data derived from similar
experiments by others.