COMPARISON OF METHANE HYDROXYLATION ACTIVITY WITH INTACT-CELLS AND CELL-FREE-EXTRACTS OF METHYLOSINUS-TRICHOSPORIUM OB3B

The number of active sites for methane hydroxylation of Methylosinus t richosporium OB3b was determined by using the inhibition method. The a ctivity per active site of methane hydroxylation with the intact cells and the cell-free extracts were compared. From the turnover numbers o f both systems, the reaction mechanism is discussed.