THERMOSTABILITY AND ELECTRON-TRANSFER ACTIVITY OF THE FERREDOXIN FROMA THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII

Authors
AONO S FUKUDA N OKURA I
Citation
S. Aono et al., THERMOSTABILITY AND ELECTRON-TRANSFER ACTIVITY OF THE FERREDOXIN FROMA THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII, Journal of molecular catalysis. A, Chemical, 95(2), 1995, pp. 173-178
Citations number
19
Categorie Soggetti
Chemistry Physical
Journal title
Journal of molecular catalysis. A, Chemical → ACNP
ISSN journal
13811169
Volume
95
Issue
2
Year of publication
1995
Pages
173 - 178
Database
ISI
SICI code
1381-1169(1995)95:2<173:TAEAOT>2.0.ZU;2-L
Abstract
The Azotobacter-type 7Fe ferredoxin from Bacillus schlegelii was activ e as an electron carrier in the reduction system of cyt. c consisting of NADPH, FNR and cytochrome c (cyt, c). The only [3Fe-4S] cluster in the Fd molecule acts as the active site for the reduction of cyt, c. T he activity of the ferredoxin once increased by 10, 20 and 20% by heat treatment at 60, 80 and 90 degrees C, respectively. The increasing of the activity is caused by the interconversion of the [4Fe-4S] cluster to the [3Fe-4S] cluster with increasing of the [3Fe-4S] cluster in th e concentration.