S. Aono et al., THERMOSTABILITY AND ELECTRON-TRANSFER ACTIVITY OF THE FERREDOXIN FROMA THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII, Journal of molecular catalysis. A, Chemical, 95(2), 1995, pp. 173-178
The Azotobacter-type 7Fe ferredoxin from Bacillus schlegelii was activ
e as an electron carrier in the reduction system of cyt. c consisting
of NADPH, FNR and cytochrome c (cyt, c). The only [3Fe-4S] cluster in
the Fd molecule acts as the active site for the reduction of cyt, c. T
he activity of the ferredoxin once increased by 10, 20 and 20% by heat
treatment at 60, 80 and 90 degrees C, respectively. The increasing of
the activity is caused by the interconversion of the [4Fe-4S] cluster
to the [3Fe-4S] cluster with increasing of the [3Fe-4S] cluster in th
e concentration.