A BRADYKININ ANTAGONIST INHIBITED NITRIC-OXIDE GENERATION AND THROMBOXANE BIOSYNTHESIS IN ACUTE-PANCREATITIS

The effect of bradykinin on nitric oxide generation and eicosanoid pro duction in the early stage of an experimental model of acute necrotizi ng pancreatitis induced by sodium taurocholate has been evaluated. We have compared the effect of administering a long-acting bradykinin ant agonist, HOE 140, and an inhibitor of nitric oxide synthase, N-G-nitro -L-arginine methyl esther L-NAME) on pancreatic prostanoid synthesis. Plasma lipase levels were increased after acute pancreatitis induction , and reduced after HOE 140 or L-NAME administration. Nitric oxide pro duction and thromboxane B-2 levels were increased after pancreatitis i nduction and the increases were reduced by L-NAME or HOE 140 administr ation. In contrast, increased prostacyclin production, reflected as 6- keto-PGF(1 alpha) levels, was not modified by L-NAME or HOE 140. Brady kinin seems to be involved in nitric oxide and thromboxane synthesis d uring the initial phases of acute necrohemorrhagic pancreatitis.