CLONING AND CHROMOSOMAL LOCALIZATION OF A PSEUDOGENE CORRESPONDING TOA MESSENGER-RNA FOR A SOLUBLE IL-6 RECEPTOR

Authors
SNYERS L RENAULD JC SZPIRER C CONTENT J
Citation
L. Snyers et al., CLONING AND CHROMOSOMAL LOCALIZATION OF A PSEUDOGENE CORRESPONDING TOA MESSENGER-RNA FOR A SOLUBLE IL-6 RECEPTOR, DNA sequence, 5(5), 1995, pp. 311-314
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology","Genetics & Heredity
Journal title
DNA sequence → ACNP
ISSN journal
10425179
Volume
5
Issue
5
Year of publication
1995
Pages
311 - 314
Database
ISI
SICI code
1042-5179(1995)5:5<311:CACLOA>2.0.ZU;2-Z
Abstract
A polymerase chain reaction assay (Lust J.A. et al. (1992). Cytokine 4 :96-100) was used to detect a mRNA coding for a soluble IL-6 receptor in human hepatoma cells. In addition to the expected amplification pro duct, we found a sequence (SR4) which could be aligned to it with 78% identity. After cloning and sequencing a genomic 2.5-2.7 kB EcoRI frag ment containing SR4, this sequence turned out to be part of a pseudoge ne corresponding to the transmembrane domain deleted soluble IL-6 rece ptor. Screening of a panel of interspecies hybrids revealed that it ma ps to chromosome 9.