RESTORATION OF MYOINOSITOL UPTAKE BY EICOSAPENTAENOIC ACID IN HUMAN SKIN FIBROBLASTS CULTURED IN HIGH-GLUCOSE MEDIUM

Authors
OKUDA Y SAWADA T MIZUTANI M SONE H SUZUKI S KAWAKAMI Y SOMA M SUZUKI M YAOITA H YAMASHITA K
Citation
Y. Okuda et al., RESTORATION OF MYOINOSITOL UPTAKE BY EICOSAPENTAENOIC ACID IN HUMAN SKIN FIBROBLASTS CULTURED IN HIGH-GLUCOSE MEDIUM, Life sciences, 57(5), 1995, pp. 71-74
Citations number
9
Categorie Soggetti
Biology,"Medicine, Research & Experimental","Pharmacology & Pharmacy
Journal title
Life sciences → ACNP
ISSN journal
00243205
Volume
57
Issue
5
Year of publication
1995
Pages
71 - 74
Database
ISI
SICI code
0024-3205(1995)57:5<71:ROMUBE>2.0.ZU;2-S
Abstract
The effects of elevated glucose and eicosapentaenoic acid (EPA, C20:5 omega 3) an myo-inositol uptake in human skin fibroblasts (HSF) were e valuated. Myo-inositol incorporation into HSF was dependent on an acti ve transport system via Na+-K+ ATPase activity based on the results wi th Na+ deprivation and ouabain (5 mM). Although glucose (27.5, 55 mM) inhibited 2-[H-3] myo-inositol uptake, the addition of EPA (3x10(-4) M ) prevented glucose-mediated inhibition. Since EPA decreased glucose-m ediated inhibition of myo-inositol uptake, this agent might ameliorate some of the devastating functions associated with diabetes.