Sixty two patients (mean age 45.6 years) were assessed for oral hygien
e and periodontal disease by dental examination before endoscopy. Info
rmation about-oral care, smoking, and dentures was obtained and sample
s of dental plaque collected. The presence of Helicobacter pylori in p
laque as sought by culture and polymerase chain reaction (PCR), and ga
stric antral biopsy specimens were taken for histological examination.
Although H pylori was detected in the antral specimens of 34 patients
(54%) all of the cultures of dental plaque were negative, and PCR was
only positive from the dentures of one patient. Smokers had poor oral
hygiene, visited their dentist less often, and brushed their teeth le
ss frequently. There was no correlation of H pylori gastritis with eit
her dental hygiene or periodontal disease. These results suggest that
dental plaque or dentures are not an important reservoir for H pylori
and are probably not a significant factor in transmission of the organ
ism. The conflicting results in published works may be caused by diffe
rences in sample collection, culture techniques, or oral contamination
from gastric juice as a result of gastro-oesophageal reflux at the ti
me of endoscopy.