NDR1, A LOCUS OF ARABIDOPSIS-THALIANA THAT IS REQUIRED FOR DISEASE RESISTANCE TO BOTH A BACTERIAL AND A FUNGAL PATHOGEN

Citation
Ks. Century et al., NDR1, A LOCUS OF ARABIDOPSIS-THALIANA THAT IS REQUIRED FOR DISEASE RESISTANCE TO BOTH A BACTERIAL AND A FUNGAL PATHOGEN, Proceedings of the National Academy of Sciences of the United Statesof America, 92(14), 1995, pp. 6597-6601
Citations number
44
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
92
Issue
14
Year of publication
1995
Pages
6597 - 6601
Database
ISI
SICI code
0027-8424(1995)92:14<6597:NALOAT>2.0.ZU;2-F
Abstract
We have employed Arabidopsis thaliana as a model host plant to genetic ally dissect the molecular pathways leading to disease resistance, A. thaliana accession Col-O is susceptible to the bacterial pathogen Pseu domonas syringae pv, tomato strain DC3000 but resistant in a race-spec ific manner to DC3000 carrying any one of the cloned avirulence genes avrB, avrRpm1, avrRpt2, and avrPph3. Fast-neutron-mutagenized Col-O M( 2) seed was screened to identify mutants susceptible to DC3000(avrB). Disease assays and analysis of in planta bacterial growth identified o ne mutant, ndr1-1 (non-race-specific disease resistance), that was sus ceptible to DC3000 expressing any one of the four avirulence genes tes ted. Interestingly, a hypersensitive-like response was still induced b y several of the strains. The ndr1-1 mutation also rendered the plant susceptible to several avirulent isolates of the fungal pathogen Peron ospora parasitica. Genetic analysis of ndr1-1 demonstrated that the mu tation segregated as a single recessive locus, located on chromosome I II. Characterization of the ndr1-1 mutation suggests that a common ste p exists in pathways of resistance to two unrelated pathogens.