SEROLOGICAL ANALYSIS OF MONOCLONAL ANTI-E AND -E WITH JAPANESE E E VARIANT RED-CELLS/

The agglutination patterns have been analyzed for the reaction between 24 monoclonal antibodies (MAB) with specificity for the Rh antigen E or e and red cells of E/e variant from Japanese blood donors. The MABs were tested for direct agglutination of papain treated cells at 20 de grees C. The reactions of a full titration series of each MAB with a E /e variant cell were compared to the reactions of that MAB with normal E+e+ cells. Sixteen anti-E were divided into a minimum of 6 different agglutination patterns with 8 examples of E variant cells. Eight anti -e gave a minimum of 5 different agglutination patterns with 6 example s of e variant cells.