DNA-FINGERPRINTING BY PULSED-FIELD GEL-ELECTROPHORESIS TO INVESTIGATEA NOSOCOMIAL PNEUMONIA CAUSED BY LEGIONELLA-BOZEMANII SEROGROUP-1

Authors
LUCK PC HELBIG JH HAGEDORN HJ EHRET W
Citation
Pc. Luck et al., DNA-FINGERPRINTING BY PULSED-FIELD GEL-ELECTROPHORESIS TO INVESTIGATEA NOSOCOMIAL PNEUMONIA CAUSED BY LEGIONELLA-BOZEMANII SEROGROUP-1, Applied and environmental microbiology, 61(7), 1995, pp. 2759-2761
Citations number
12
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
7
Year of publication
1995
Pages
2759 - 2761
Database
ISI
SICI code
0099-2240(1995)61:7<2759:DBPGTI>2.0.ZU;2-Z
Abstract
We typed 18 isolates of Legionella bozemanii obtained from clinical an d environmental sources by pulsed-field gel electrophoresis. Each of t he unrelated strains showed individual restriction patterns of the gen omic DNA when either the SfiI or NotI restriction enzyme was used. One strain isolated from a patient with nosocomial legionellosis and two strains from the corresponding hospital water supply were indistinguis hable, arguing for a transmission of L. bozemanii from the water suppl y to the patient. In conclusion, macrorestriction analysis is a valuab le tool for studies of the molecular epidemiology of L. bozemanii.