DIRECT MEASUREMENTS OF NATURAL PLANKTONIC BACTERIAL COMMUNITY VIABILITY BY FLOW-CYTOMETRY

Authors
PORTER J DIAPER J EDWARDS C PICKUP R
Citation
J. Porter et al., DIRECT MEASUREMENTS OF NATURAL PLANKTONIC BACTERIAL COMMUNITY VIABILITY BY FLOW-CYTOMETRY, Applied and environmental microbiology, 61(7), 1995, pp. 2783-2786
Citations number
26
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
7
Year of publication
1995
Pages
2783 - 2786
Database
ISI
SICI code
0099-2240(1995)61:7<2783:DMONPB>2.0.ZU;2-4
Abstract
A range of fluorescent viability dyes were used in conjunction with fl ow cytometry to rapidly enumerate viable bacteria from freshwater envi ronments. Optimal labelling was achieved by using carboxyfluorescein d iacetate or chemchrome B with a detergent-mediated permeabilization st ep. The viable bacterial count under optimal conditions was 7% in olig otrophic lake water and 75% in polluted river water.