PCR DETECTION OF THE LACTOCIN-S STRUCTURAL GENE IN BACTERIOCIN-PRODUCING LACTOBACILLI FROM MEAT

Authors
RODRIGUEZ JM CINTAS LM CASAUS P SUAREZ A HERNANDEZ PE
Citation
Jm. Rodriguez et al., PCR DETECTION OF THE LACTOCIN-S STRUCTURAL GENE IN BACTERIOCIN-PRODUCING LACTOBACILLI FROM MEAT, Applied and environmental microbiology, 61(7), 1995, pp. 2802-2805
Citations number
36
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
7
Year of publication
1995
Pages
2802 - 2805
Database
ISI
SICI code
0099-2240(1995)61:7<2802:PDOTLS>2.0.ZU;2-F
Abstract
The lactocin S structural gene (lasA) in seven bacteriocinogenic lacto bacilli isolated from fermented sausages was studied. Two degenerate p rimers were synthesized to amplify a 75-bp fragment of the gene. Three strains amplified the fragment from their plasmid DNA, and hybridizat ion analysis confirmed these results.