SUPPRESSION OF BIOLUMINESCENCE OF HUMAN-L EUKOCYTES IN-VITRO UNDER SENSITIZED PHOTOLYSIS

Luminol dependent bioluminescence of polymorphonuclear neutrophil leuk ocytes (PMNL) in vitro has been shown to cease completely during a few minutes after the dye sensitized cell photolysis under the same condi tions Of irradiation (the photosensitizer and its concentration, the i ntensity and spectral distribution of incident light, irradiation time and temperature) as used in the photodynamic therapy. of cancer (PDT) . Usually a violation. of carcinoma and normal cells due to PDT is det ected after 20-24 hours. So the effect of complete suppression of biol uminescence is the earliest detectable manifestation of cell damage by light. Bioluminescence of cells is repaired by the addition of Mg2+ i ons to the human leukocyte suspension irradiated. The data obtained on bioluminescence of leukocytes and the effect of Mg2+ ions exhibit the violation of enzyme system under the sensitized cell photolysis.