FLUORESCENT DIAGNOSTICS OF RAT-BLOOD LYMP HOCYTES UNDER CONTINUOUS GAMMA-IRRADIATION

Changes of parameter a of rat blood lymphocytes under continuous gamma -irradiation at the dose rate 14.4 cGy/day have been investigated by f luorescence microspectral analysis. Parameter alpha reflects the cell synthetic activity and represents the ratio of intensities of the red (640 nm) and green (530 nm) components the fluorescence spectrum of ac ridine orange fluorochromed cells. It has been demonstrated that chang es of alpha-parameter occurs in two stages: 1)- the sharp increase wit h the maximum at 3th-7th day (accumulated dose 0.5-1.0 Gy), 2) fluctua tion near the control level with secondary increase of alpha-parameter at 45th-63th day (accumulated dose 6-9 Gy). At each stage there exist typical specific shapes of parameter alpha distribution histogram. It is discussed that continuos gamma-irradiation induced both adaptive r eaction of organism on the first stage and damaging effect on immune s ystem cells on the second stage.