SYNTHESIS OF 3,4-DIMETHOXYPHENYL BETA-D-GLYCOPYRANOSIDE AND ITS RELATED GLYCOSIDES BY CULTURED PLANT-CELLS

3,4-Dimethoxyphenol (3,4-DMP) was converted into the corresponding glu coside in suspension-cultured cells of Coffea arabica. The maximum eff iciency of glucosylation was attained, more than 40%, within 96 h afte r the addition of 1 mM 3,4-DMP when cultured in a modified Murashige a nd Skoog's medium with 5 mu M 2,4-dichlorophenoxyacetic acid and 0.5 m u M kinetin, The glucoside was identified as 3,4-di-methoxyphenyl beta -D-glucopyranoside (3,4-DMP glucoside) by H-1-NMR and hydrolysis with alpha- and beta-glucosidases, As the application of the glycosylation by C. arabica cells, eleven glycosides were obtained from seven methox yphenols including 3,4-DMP.