SOME PROPERTIES OF TRANSGLYCOSYLATION ACTIVITY OF SESAME BETA-GLUCOSIDASE

Authors
KURIYAMA K TSUCHIYA K MURUI T
Citation
K. Kuriyama et al., SOME PROPERTIES OF TRANSGLYCOSYLATION ACTIVITY OF SESAME BETA-GLUCOSIDASE, Bioscience, biotechnology, and biochemistry, 59(6), 1995, pp. 1142-1143
Citations number
7
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
59
Issue
6
Year of publication
1995
Pages
1142 - 1143
Database
ISI
SICI code
0916-8451(1995)59:6<1142:SPOTAO>2.0.ZU;2-G
Abstract
The transglycosylation reaction of partially purified beta-glucosidase from sesame seeds with cellobiose is described. Sesame beta-glucosida se was partially purified by ammonium sulfate fractionation and gel fi ltration. The molecular weight of the enzyme was 200,000 by gel filtra tion. Sesame beta-glucosidase showed strong transfer activity to synth esize the trisaccharide from cellobiose. The optimum pH and temperatur e of the transglycosylation reaction were pH 4.0 and 60 degrees C.