A. Pukhalsky et al., INTERLEUKIN-2 RECEPTOR-BETA CHAIN AS A POSSIBLE TARGET FOR LOW-DOSES OF MAFOSFAMIDE, Mediators of inflammation, 4(3), 1995, pp. 175-180
THE 7-day cytotoxic lymphocytes (CTL) induced in mixed lymphocyte cult
ure express only the beta chain of the interleukin-a receptor (IL-2R).
In the present study this fact has been confirmed in a murine semi-al
logeneic system. The ability of low doses of mafosfamide (Mf) to affec
t IL-2-induced CTL proliferation has been demonstrated. It was also sh
own that IL-2 activated resting suppressor cells. The pretreatment of
the suppressor cells with either monoclonal antibodies (mAbs) against
the p75 chain of IL-2R, or with Mf abolished the suppressive effect of
these cells. No restoration of the proliferative response occurred wh
en the anti-IL-2R alpha mAb had been used. Flow cytometry analysis of
7-day CTL was carried out with mAbs against the alpha and beta chains
of IL-2R CTL treatment with Mf inhibited anti-IL-2R beta mAb binding.
It may be assumed that the antiproliferative effects of Mf which have
been demonstrated in this paper, were a result of blocking the IL-2R b
eta chain.