STUDIES OF MYOCARDIAL WORK AND METABOLIC STRESS BY P-31 NMR-SPECTROSCOPY

Tile application of the P-31 NMR spectroscopy is described for the stu dy of phosphorus containing. compounds, pH and metabolite fluxes throu gh key enzymes in high energy phosphates metabolism of myocardium in p hysiological and pathological conditions both in animals and humans. T he main interest was focused on transmural differences in metabolites in the myocardium, and biochemical changes which call be studied by th is method in hypoxia, anoxia, ischaemia, ventricular fibrillation, myo cardial hypertrophy and hibernation. The main advantage of this method is the possibility to study myocardial metabolism in real time non-in vasively and non-destructively. It can be concluded that P-31 NMR spec troscopy is a modern method which allowed the study of the myocardium in vitro, in situ and in vivo with a great potential for further appli cation in experimental work and clinical practice.