U937 CELL-LINE HAS A T(1O-11)(P13-14-Q14-21) RATHER THAN A DELETION OF 11Q

The U937 cell line was studied with the fluorescence in situ hybridiza tion (FISH) technique using phage and cosmid probes which were mapped and ordered on 11q. Although this cell line was thought to have a del( 11q), FISH demonstrated that 11q was translocated to 10p and that the breakpoint on 11q is centromeric to the MLL gene. This 10;11 transloca tion appears to be a t(10;11)(p13-14;q14-21), which was recently repor ted to be a recurring translocation in malignant hematologic disease. This cell line will be a good tool for the study of this chromosomal r earrangement.