AMINO-ACID-SEQUENCE OF A MODIFIED BETA-2-MICROGLOBULIN IN RENAL-FAILURE PATIENT URINE AND LONG-TERM DIALYSIS PATIENT BLOOD

We isolated and analyzed beta 2-microglobulin (BM) in urine of two ren al failure patients and in ultrafiltrate from long-term dialysis patie nts, In both groups of patients, we found the native BM with pI 5.7 an d some modified BM with pI 5.3. The amino acid sequence of the modifie d BM was determined and compared with that of the native BM; the N-ter minal isoleucine residue was missing and the second glutamine residue was cyclized into pyroglutamic acid in the modified BM.