GLYCOSYLATED HUMAN PROLACTIN - DETECTION BY IMMUNOBLOTTING OF BIOLOGICAL-FLUIDS IS CONFOUNDED BY ANTISERA CROSS-REACTIVITY WITH IMMUNOGLOBULINS

Glycosylated human prolactin is found in several biological fluids and can only be detected by western immunoblotting. Glycosylated prolacti n migrates at 25,000-27,000 molecular weight while prolactin migrates at 23,000. We have discovered that several antihuman prolactin sera cr ossreact with some light chain components of human immunoglobulins, wh ich also migrate at 25,000 on electrophoretic gels run under reducing conditions. Evidence is provided for prolactin binding to immunoglobul ins in biological fluids, which may explain why some polyclonal anti-p rolactin sera demonstrate this crossreactivity. (C) 1995 Academic Pres s, Inc.