DYNAMIC CHANGES IN HEMATOLOGICAL PARAMETE RS IN THE BLOOD OF SHEEP ATESTRUS SYNCHRONIZATION AND IN SUBSEQUENT PERIOD OF EARLY-PREGNANCY

Observation of hematological indices which are a reflection of physiol ogical processes in the individual phases of the reproductive cycle, p resents one of the methods of controlling the homeostasis of the ewe's organism. This work focused on the observation of selected indices du ring synchronization treatment and in the first days of pregnancy. The experiment included 10 conventionally bred animals. Blood was obtaine d from the v. jugularis into an oxalate mixture on days 0 (date of syn chronization), 3, 7 and 14 of Agelin implantation, on the day of oestr us and days 7, 14, 17 and 34 of pregnancy. Erythrocyte counts were det ermined by the Burker flask method, hemoglobin concentrations by spect rophotometry and packed cell volume (PCV) by the method of Janetzki. F or leucocyte count determination the Burker flask method with Turk's s olution was used; the percentual proportion of leucocytes was stated b y the leucogram. During synchronization treatment and pregnancy the me an erythrocyte counts ranged from 9.16 +/- 0.81 to 9.74 +/- 0.61 T/l a nd from 9.09 +/- 0.84 to 9.47 +/- 1.11 T/l, respectively. Hemoglobin c oncentrations oscillated between 10.71 +/- 1.02 and 11.83 +/- 1.01 g/l . PCV values reached between 32.44 +/- 2.18 and 39.33 +/- 2.73 l/l wit h a significant increase on day 14 of Agelin implantation and days 17 and 34 of pregnancy (P < 0.05). This dynamics of the red blood compone nts points to the occurrence of functional-morphological changes in th e sexual apparatus during oestrus and Agelin treatment. Through mediat ion by the vascular system a change in the mean values may also occur (Kresan et al., 1979). Leucocyte counts did not surpass the reference values but revealed a significant decrease (P < 0.01) on day 34 of pre gnancy (5.14 +/- 1.39 G/l). Neutrophilic segments, eosinophilic granul ocytes, lymphocytes and monocytes were present at counts ranging from 23.25 +/- 12.72 to 37.77 +/- 11.15, from 5.25 +/- 3.22 to 7.75 +/- 2.9 1, from 56.22 +/- 12.14 to 67.77 +/- 7.90 and from 0 to 2.25 +/- 1.02, respectively. If on the basis of literary data (Kudlac, Elecko et al. , 1977) we suppose that, within the progestational changes, leucocytes participate in the biological protection of the organism during forma tion of the pre-implantational environment and implantation, then our results seem to be comparable with those of the above authors and, for the investigated period, confirm the role of leucocytes in the defenc e abilities of the ewe's organism.