THE HUMAN GONADOTROPIN-RELEASING-HORMONE RECEPTOR GENE (GNRHR) MAPS TO CHROMOSOME BAND 4Q13

A cDNA representing the high-affinity gonadotropin-releasing hormone ( GnRH) receptor has been molecularly cloned from the human pituitary gl and, a breast tumor cell line (MCF 7), and an ovarian tumor. The nucle otide sequence of this cDNA was determined, and its expression in vari ous human tumors and tumor cell lines was demonstrated. In this study, we localized the gene encoding the GnRH receptor to human chromosome 4, using polymerase chain reaction (PCR) analysis of genomic DNA from human x hamster somatic cell hybrids. The gene was sublocalized to chr omosome band 4q13 using fluorescence in situ hybridization with the Gn RH receptor gene (GNRHR).