CROSS-RESISTANCE TO POLYENE AND AZOLE DRUGS IN CRYPTOCOCCUS-NEOFORMANS

Fluconazole was observed to inhibit sterol 14 alpha-demethylase in the human pathogen Cryptococcus neoformans, and accumulation of a ketoste roid product was associated with growth arrest. A novel mechanism(s) o f azole and amphotericin B cross-resistance was identified, unrelated to changes in sterol biosynthesis, as previously identified in Sacchar omyces cerevisiae, Reduced cellular content of drug could account for the resistance phenotype, indicating the possible involvement of a mec hanism similar to multidrug resistance observed in higher eukaryotes.