REGULATION OF HUMAN-LEUKOCYTE P21-ACTIVATED KINASES THROUGH G-PROTEIN-COUPLED RECEPTORS

Citation
Ug. Knaus et al., REGULATION OF HUMAN-LEUKOCYTE P21-ACTIVATED KINASES THROUGH G-PROTEIN-COUPLED RECEPTORS, Science, 269(5221), 1995, pp. 221-223
Citations number
31
Categorie Soggetti
Multidisciplinary Sciences
Journal title
ISSN journal
00368075
Volume
269
Issue
5221
Year of publication
1995
Pages
221 - 223
Database
ISI
SICI code
0036-8075(1995)269:5221<221:ROHPKT>2.0.ZU;2-E
Abstract
The Rac guanosine 5'-triphosphate (GTP)-binding proteins regulate oxid ant production by phagocytic leukocytes. Two Ste20-related p21-activat ed kinases (PAKs) were identified as targets of Rac in human neutrophi ls. Activity of the similar to 65- and similar to 68-kilodalton PAKs w as rapidly stimulated by chemoattractants acting through pertussis tox in-sensitive heterotrimeric GTP-binding proteins (G proteins). Native and recombinant PAKs phos phorylated the p47(phox) reduced nicotinamid e adenine dinucleotide phosphate (NADPH) oxidase component in a Rac-GT P-dependent manner. The action of PAKs during phagocyte activation by G protein-coupled pathways may contribute to regulation of NADPH oxida se activity.