H. Shirwan et al., DIFFERENTIAL USAGE OF THE T-CELL RECEPTOR REPERTOIRE FOR ALLORECOGNITION OF HEART, LIVER, AND KIDNEY GRAFTS, Transplantation, 59(12), 1995, pp. 1709-1714
We have previously demonstrated that the immune response to cardiac al
lografts in the ACI-to-LEW rat strain combination involves a limited u
se of the TCR V beta gene repertoire. In the present study we analyzed
the expression of V beta genes by T cells infiltrating kidney and liv
er allografts to test whether a limited use of the T cell receptor (TC
R) repertoire is a common denominator for immune responses to allograf
ts. Graft-infiltrating lymphocytes (GIL) were isolated from allografts
on different days after transplantation and analyzed for the expressi
on of V beta genes using a semiquantitative polymerase chain reaction
(PCR) without manipulations in tissue culture. We detected a limited e
xpression of the V beta gene repertoire in fresh GIL harvested from bo
th kidney and liver allografts early in graft rejection. The level of
TCR repertoire usage, however, was influenced by the type of graft. Th
e rejection of heart and kidney allografts was associated with more li
mited use of the V beta gene repertoire when compared with that seen f
or the rejection of liver allografts. The limited use of the V beta ge
ne repertoire was only apparent when analyzed early in graft rejection
; as the rejection reaction progressed T cells using a more diverse V
beta repertoire infiltrated the graft. The limited use of TGR repertoi
re of the early T cell response to allografts may provide the opportun
ity to therapeutically disrupt the rejection reaction by targeting sel
ected T cell populations for elimination at the time of organ transpla
ntation.