Dr. Boone et al., BACILLUS-INFERNUS SP-NOV, AN FE(III)-REDUCING AND MN(IV)-REDUCING ANAEROBE FROM THE DEEP TERRESTRIAL SUBSURFACE, International journal of systematic bacteriology, 45(3), 1995, pp. 441-448
Bacillus infernus sp, nov. was isolated from ca, 2,700 m below the lan
d surface in the Taylorsville Triassic Basin in Virginia. B. infernus
was a strict anaerobe that grew on formate or lactate with Fe(III), Mn
O2, trimethylamine oxide, or nitrate (reduced to nitrite) as an electr
on acceptor, and it also grew fermentatively on glucose, Type strain T
H-23 and five reference strains were gram-positive rods that were ther
mophilic (growth occurred at 61 degrees C), halotolerant (good growth
occurred in the presence of Na+ concentrations up to 0.6 M), and very
slightly alkaliphilic (good growth occurred at pH 7.3 to 7.8). A phylo
genetic analysis of its 16S rRNA indicated that B. infernus should be
classified as a new species of the genus Bacillus. B. infernus is the
only strictly anaerobic species in the genus Bacillus.